1. Department Of Biomedical Science

Determination of antimicrobial activity of Rhipsalisbaccifera and Pyrrosiaheterophylla against MRSA and Acinetobacter baumanii.

SLCM-2019.pdf

Introduction

Finding alternative antimicrobials for the emerging antimicrobial resistance is a global challenge. Traditional dressings made of Rhipsalis baccifera and Pyrrosia heterophylla have been used in traditional practice to treat woundsin Sri Lanka. However, their antimicrobial properties have not been scientifically investigated. This study investigates the antimicrobial activity of these two plant extracts against MRSA and Acinetobacter baumanii responsible for acute and chronic wound infections.

Objective

To determine the antibacterial activity of Rhipsalis baccifera and Pyrrosia heterophylla against MRSA and Multi drug resistant Acinetobacter baumanii.

Design

A descriptive study

Setting

Student laboratory, KIU.

Methodology

Clinical isolates of MRSA and Acinetobacter baumanii were obtained from the Department of Microbiology, Faculty of Medicine, University of Sri Jayawardanapura. The entire plant of Rhipsalis baccifera and leaves of Pyrrosia heterophylla was obtained from Beruwala, Sri Lanka. Aqueous extractions of both plants were done by maceration of 120g of wet plant material with 1440ml of distilled water and filter sterilized using a 0.22µm filter. This was then boiled to 240ml and this is taken as ×1 concentration and this was further concentrated as ×2, ×3, ×5, ×10 respectively. Antimicrobial activity of the extracts against MRSA and Acinetobacter baumanii was determined by well diffusion method.

Results

Rhipsalis baccifera demonstrated mean zones of inhibition of diameter 9mm, 11mm, 13mm, 14mm, 16mm for the concentrations ×1, ×2, ×3, ×5, ×10 respectively when tested against MRSA. No zones of inhibition were observed against Acinetobacter baumanii. Pyrrosia heterophylla did not demonstrate any inhibitory zones against both MRSA and Acinetobacter baumanii.

Conclusion

Rhipsalis baccifera leaf extract has potential antimicrobial activity against MRSA. Further studies should be carried out to identify the phytochemicals responsible for this activity and to extend the study to determine antimicrobial activity against other common wound pathogens.

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