KDU-2019.pdf

Abstract Discovery of novel antimicrobials is a necessity due to the emerging antimicrobial resistance. Medicinal plants are a rich source for discovering novel antimicrobial compounds. Traditional dressings made of Madhuca longifolia (mee)has been used in traditional medicinal practices in Sri Lanka to treat wounds, but its antimicrobial properties have not been scientifically investigated. The objective of this study was to investigate the antimicrobial activity of this plant extract against Methicillin Resistance staphylococcus aureus(MRSA) and Acinetobacter baumanii which are known to infect both acute and chronic wounds. This is a descriptive study performed at student laboratory, KIU.

Two clinical isolates of MRSA and Acinetobacter baumanii were obtained from the Department of Microbiology, Faculty of Medicine, University of Sri Jayawardanapura. Madhuca longifolia (mee) was obtained from Beruwala area in Sri Lanka. Aqueous extraction of the plant was prepared by maceration of 60g of dried leaves with 1440ml of distilled water followed by filter sterilization using 0.22um filter. This was then boiled down to 240ml which was labelled as x1 the concentration. This was further concentrated as ×2, ×3, ×5, ×10. Antimicrobial activity of these extracts were determined by well diffusion method.

The mean zones of inhibition against MRSA were 18mm, 16.7mm, 15.6mm, 11.6mm for the concentrations ×1, ×2, ×5, ×10 respectively. No inhibition zones were observed against Acinetobacter baumanii.

Madhuca longifolia (mee) leaf extract has potential antimicrobial activity against MRSA. Further studies should be carried out to identify the phytochemicals responsible for this activity and to determine antimicrobial activity against other common wound pathogens.

 

Keywords: Antimicrobial, MRSA, Acinetobactor baumanii

 

 

 

 

 

 

 

Extended Abstract

Introduction:

Antimicrobial resistance poses a very serious health threat. Infections from  resistant bacteria are becoming very common, and some pathogens have gained  resistance to antibiotics, posing  a major challenge in the treatment of infections(Mahesh & Satish, 2008). The Finding alternative antimicrobials for the emerging antimicrobial resistance is a global challenge. Medicinal plants have been proven to contain constituents which provide therapeutic value and represent a rich source of antimicrobial agents(Biswas & Mukherjee, 2003). Traditional dressings made of Madhuca longifolia (mee) have been used in traditional practice to treat wounds in Sri Lanka. Although this plant is being used in traditional medicine over many years, its antimicrobial properties have not been scientifically investigated. The aim of  this study is to investigate the antimicrobial activity of this plant extract against MRSA and Acinetobacter baumanii which are known to be prominent pathogens causing both acute and chronic wound infections(Bishburg & Bishburg, 2009).

Objectives: General objective is to determine the antibacterial activity of selected medicinal plants against Methicillin Resistant Staphylococus aureus and multidrug resistant Acinetobacter baumanii.

Specific Objectives are to determine the antibacterial activity of the plant aqueous extracts against the selected bacterial isolates using agar well diffusion assay, to determine the effect of concentrations to the inhibitory zones and to determine the time duration which plant extract give its antibacterial activity by checking plant extract after one day and eight week from time of extraction, against selected bacterial isolates using agar well diffusion assay.

Methodology:

The study design is a descriptive study. Study was done in student laboratory, KIU. Clinical isolates of MRSA and Acinetobacter baumanii were obtained from the Department of Microbiology, Faculty of Medicine, University of Sri Jayawardanapura. Madhuca longifolia (mee) was obtained from Beruwala area in Sri Lanka. Aqueous extraction of the plant was done by maceration of 60g of dried leaves of the selected plant material with 1440ml of distilled water. And it was filter sterilized using 0.22um filter. Then an aqueous extract was prepared by boiling the extract till the volume reached to 240ml and the concentration of that was taken as x1. Resulted extract was concentrated as ×2, ×3, ×5, ×10. Antimicrobial activity of these all extracts against MRSA and Acinetobacter baumanii were determined by well diffusion method, day after preparing the extracts and after eight week from time of extraction.

Result and discussion:

Date: 01/10/2018 (day after preparing extract)

Bacterial agent: MRSA

Diameter of the well: 9mm

Table 1. Results of inhibitory zones day after preparing extract

Concentration factor1st trial2nd trial3rd trialMean value
Positive control25252524242424242424.3
X118181818181818181818
X217171617161717161716.7
X515161616161615151515.6
X1011111111121212121211.6

 Madhuca longifolia (mee) demonstrated no inhibitive zones for Acinetobacter baumanii.

 

 

Figure 1. inhibitory zones day after preparing extract

Date: 02/12/2018 ( two month later)

Bacterial agent: MRSA

Diameter of the well: 9mm

Table 2. Results of inhibitory zones after two month preparing extract

Concentration factor1st trial2nd trial3rd trialMean value
Positive control26262627272726262626.3
X124242425252524242424.3
X223222223232322222222.4
X520212121212121212120.9
X1020192020191920202019.7

 

Madhuca longifolia (mee) demonstrated no inhibitive zones for Acinetobacter baumanii.

 

Figure 2. inhibitory zones  after two month preparing extract

According to above results, there is an antibacterial property in Madhuca longifolia aqueous leaf extract against MRSA. When the concentration of the extract increases, size of the inhibitory zones become less.

Zone sizes are comparatively higher in two month later than day after preparing extract.

Conclusion:

Madhuca longifolia (mee) leaf extract has potential antimicrobial activity against MRSA. Further studies should be carried out to identify the phytochemicals responsible for this activity and to extend the study to determine antimicrobial activity against other common wound pathogens.

 

 

 

References:

Bishburg, E., & Bishburg, K. (2009). Minocycline—an old drug for a new century: emphasis on methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii. International journal of antimicrobial agents, 34(5), 395-401.

Biswas, T. K., & Mukherjee, B. (2003). Plant medicines of Indian origin for wound healing activity: a review. The international journal of lower extremity wounds, 2(1), 25-39.

Abstract Discovery of novel antimicrobials is a necessity due to the emerging antimicrobial resistance. Medicinal plants are a rich source for discovering novel antimicrobial compounds. Traditional dressings made of Madhuca longifolia (mee)has been used in traditional medicinal practices in Sri Lanka to treat wounds, but its antimicrobial properties have not been scientifically investigated. The objective of this study was to investigate the antimicrobial activity of this plant extract against Methicillin Resistance staphylococcus aureus(MRSA) and Acinetobacter baumanii which are known to infect both acute and chronic wounds. This is a descriptive study performed at student laboratory, KIU.

Two clinical isolates of MRSA and Acinetobacter baumanii were obtained from the Department of Microbiology, Faculty of Medicine, University of Sri Jayawardanapura. Madhuca longifolia (mee) was obtained from Beruwala area in Sri Lanka. Aqueous extraction of the plant was prepared by maceration of 60g of dried leaves with 1440ml of distilled water followed by filter sterilization using 0.22um filter. This was then boiled down to 240ml which was labelled as x1 the concentration. This was further concentrated as ×2, ×3, ×5, ×10. Antimicrobial activity of these extracts were determined by well diffusion method.

The mean zones of inhibition against MRSA were 18mm, 16.7mm, 15.6mm, 11.6mm for the concentrations ×1, ×2, ×5, ×10 respectively. No inhibition zones were observed against Acinetobacter baumanii.

Madhuca longifolia (mee) leaf extract has potential antimicrobial activity against MRSA. Further studies should be carried out to identify the phytochemicals responsible for this activity and to determine antimicrobial activity against other common wound pathogens.

 

Keywords: Antimicrobial, MRSA, Acinetobactor baumanii

 

 

 

 

 

 

 

Extended Abstract

Introduction:

Antimicrobial resistance poses a very serious health threat. Infections from  resistant bacteria are becoming very common, and some pathogens have gained  resistance to antibiotics, posing  a major challenge in the treatment of infections(Mahesh & Satish, 2008). The Finding alternative antimicrobials for the emerging antimicrobial resistance is a global challenge. Medicinal plants have been proven to contain constituents which provide therapeutic value and represent a rich source of antimicrobial agents(Biswas & Mukherjee, 2003). Traditional dressings made of Madhuca longifolia (mee) have been used in traditional practice to treat wounds in Sri Lanka. Although this plant is being used in traditional medicine over many years, its antimicrobial properties have not been scientifically investigated. The aim of  this study is to investigate the antimicrobial activity of this plant extract against MRSA and Acinetobacter baumanii which are known to be prominent pathogens causing both acute and chronic wound infections(Bishburg & Bishburg, 2009).

Objectives: General objective is to determine the antibacterial activity of selected medicinal plants against Methicillin Resistant Staphylococus aureus and multidrug resistant Acinetobacter baumanii.

Specific Objectives are to determine the antibacterial activity of the plant aqueous extracts against the selected bacterial isolates using agar well diffusion assay, to determine the effect of concentrations to the inhibitory zones and to determine the time duration which plant extract give its antibacterial activity by checking plant extract after one day and eight week from time of extraction, against selected bacterial isolates using agar well diffusion assay.

Methodology:

The study design is a descriptive study. Study was done in student laboratory, KIU. Clinical isolates of MRSA and Acinetobacter baumanii were obtained from the Department of Microbiology, Faculty of Medicine, University of Sri Jayawardanapura. Madhuca longifolia (mee) was obtained from Beruwala area in Sri Lanka. Aqueous extraction of the plant was done by maceration of 60g of dried leaves of the selected plant material with 1440ml of distilled water. And it was filter sterilized using 0.22um filter. Then an aqueous extract was prepared by boiling the extract till the volume reached to 240ml and the concentration of that was taken as x1. Resulted extract was concentrated as ×2, ×3, ×5, ×10. Antimicrobial activity of these all extracts against MRSA and Acinetobacter baumanii were determined by well diffusion method, day after preparing the extracts and after eight week from time of extraction.

Result and discussion:

Date: 01/10/2018 (day after preparing extract)

Bacterial agent: MRSA

Diameter of the well: 9mm

Table 1. Results of inhibitory zones day after preparing extract

Concentration factor1st trial2nd trial3rd trialMean value
Positive control25252524242424242424.3
X118181818181818181818
X217171617161717161716.7
X515161616161615151515.6
X1011111111121212121211.6

 Madhuca longifolia (mee) demonstrated no inhibitive zones for Acinetobacter baumanii.

 

 

Figure 1. inhibitory zones day after preparing extract

Date: 02/12/2018 ( two month later)

Bacterial agent: MRSA

Diameter of the well: 9mm

Table 2. Results of inhibitory zones after two month preparing extract

Concentration factor1st trial2nd trial3rd trialMean value
Positive control26262627272726262626.3
X124242425252524242424.3
X223222223232322222222.4
X520212121212121212120.9
X1020192020191920202019.7

 

Madhuca longifolia (mee) demonstrated no inhibitive zones for Acinetobacter baumanii.

 

Figure 2. inhibitory zones  after two month preparing extract

According to above results, there is an antibacterial property in Madhuca longifolia aqueous leaf extract against MRSA. When the concentration of the extract increases, size of the inhibitory zones become less.

Zone sizes are comparatively higher in two month later than day after preparing extract.

Conclusion:

Madhuca longifolia (mee) leaf extract has potential antimicrobial activity against MRSA. Further studies should be carried out to identify the phytochemicals responsible for this activity and to extend the study to determine antimicrobial activity against other common wound pathogens.

 

 

 

References:

Bishburg, E., & Bishburg, K. (2009). Minocycline—an old drug for a new century: emphasis on methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii. International journal of antimicrobial agents, 34(5), 395-401.

Biswas, T. K., & Mukherjee, B. (2003). Plant medicines of Indian origin for wound healing activity: a review. The international journal of lower extremity wounds, 2(1), 25-39.

Mahesh, B., & Satish, S. (2008). Antimicrobial activity of some important medicinal plant against plant and human pathogens. World journal of agricultural sciences, 4(5), 839-843.

 

, B., & Satish, S. (2008). Antimicrobial activity of some important medicinal plant against plant and human pathogens. World journal of agricultural sciences, 4(5), 839-843.

 

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